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human et 1 quantikine elisa kit  (R&D Systems)


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    R&D Systems human et 1 quantikine elisa kit
    Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by <t>ELISA</t> (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.
    Human Et 1 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human et 1 quantikine elisa kit/product/R&D Systems
    Average 99 stars, based on 184 article reviews
    human et 1 quantikine elisa kit - by Bioz Stars, 2026-03
    99/100 stars

    Images

    1) Product Images from "Spermidine from arginine metabolism activates Nrf2 and inhibits kidney fibrosis."

    Article Title: Spermidine from arginine metabolism activates Nrf2 and inhibits kidney fibrosis.

    Journal: Communications biology

    doi: 10.1038/s42003-023-05057-w

    Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by ELISA (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.
    Figure Legend Snippet: Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by ELISA (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction, Incubation, Staining



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    Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by <t>ELISA</t> (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.
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    Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by <t>ELISA</t> (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.
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    Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by <t>ELISA</t> (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.
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    Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by <t>ELISA</t> (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.
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    Image Search Results


    Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by ELISA (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.

    Journal: Communications biology

    Article Title: Spermidine from arginine metabolism activates Nrf2 and inhibits kidney fibrosis.

    doi: 10.1038/s42003-023-05057-w

    Figure Lengend Snippet: Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by ELISA (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.

    Article Snippet: To measure TGFβ1 and ET-1 concentrations in the supernatant, we used the human TGFβ1 Quantikine ELISA kit (DB100B; R&D Systems, Minneapolis, MN, USA) and the human ET-1 Quantikine ELISA kit (DET100; R&D Systems).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction, Incubation, Staining